TY - JOUR
T1 - Analysis of interleukin-10 anti-inflammatory cytokines in salivary lymphocyte surface
T2 - A cross sectional study
AU - Indrawati, Retno
AU - Luthfi, Muhammad
AU - Oki, Aqsa
AU - Yuliati, Y.
AU - Sosiawan, Agung
AU - Rachmadi, Priyawan
AU - Rifai, Muhaimin
N1 - Funding Information:
The authors would like to thank Directorate of Research and Community Services of Directorate General of Research and Development Strengthening from Ministry of Research, Technology and Higher Education of the Republic of Indonesia for the grant funding provided for this research.
Publisher Copyright:
© 2020 Journal of International Oral Health | Published by Wolters Kluwer - Medknow.
PY - 2020/9/1
Y1 - 2020/9/1
N2 - Aim: To analyze the expression of interleukin-10 (IL-10) in children with severe early childhood caries (S-ECC) and caries-free children. Materials and Methods: This was an observational analytic pilot study performed on children with social factors-ECC (S-ECC), and caries-free children as the objects of research with a cross-sectional study design. Saliva of children aged 4-6 years from the group of caries children in severe and caries-free early childhood was taken. Samples were taken by rinsing with 1.5% sterile NaCl for 30s and then accommodated in a sterile tube, to get a 40 mL sample from the aforementioned procedure repeated four times. Flow-cytometry test was used to analyze the IL-10 expression. The results of the study were analyzed using the normality test using Shapiro-Wilk, then continued with t test using the Statistical Package for the Social Sciences (SPSS) software program, version 20.0 (IBM Corp., Armonk, NY, USA). The data were analyzed by independent t test to see the difference between caries-free children and S-ECC. Results: The expression of IL-10 in the saliva of children with severe ECC was 3.32 ± 0.79; meanwhile, in caries-free children it was 4.04 ± 0.65. Conclusion: The IL-10 expression in children with severe ECC was significantly lower than that of in caries-free children.
AB - Aim: To analyze the expression of interleukin-10 (IL-10) in children with severe early childhood caries (S-ECC) and caries-free children. Materials and Methods: This was an observational analytic pilot study performed on children with social factors-ECC (S-ECC), and caries-free children as the objects of research with a cross-sectional study design. Saliva of children aged 4-6 years from the group of caries children in severe and caries-free early childhood was taken. Samples were taken by rinsing with 1.5% sterile NaCl for 30s and then accommodated in a sterile tube, to get a 40 mL sample from the aforementioned procedure repeated four times. Flow-cytometry test was used to analyze the IL-10 expression. The results of the study were analyzed using the normality test using Shapiro-Wilk, then continued with t test using the Statistical Package for the Social Sciences (SPSS) software program, version 20.0 (IBM Corp., Armonk, NY, USA). The data were analyzed by independent t test to see the difference between caries-free children and S-ECC. Results: The expression of IL-10 in the saliva of children with severe ECC was 3.32 ± 0.79; meanwhile, in caries-free children it was 4.04 ± 0.65. Conclusion: The IL-10 expression in children with severe ECC was significantly lower than that of in caries-free children.
KW - Interleukin-10 Anti-inflammatory Cytokines
KW - Lymphocyte Cells
KW - Severe Early Childhood Caries
UR - http://www.scopus.com/inward/record.url?scp=85095720915&partnerID=8YFLogxK
U2 - 10.4103/jioh.jioh_293_18
DO - 10.4103/jioh.jioh_293_18
M3 - Article
AN - SCOPUS:85095720915
VL - 12
SP - 439
EP - 442
JO - Journal of International Oral Health
JF - Journal of International Oral Health
SN - 0976-7428
IS - 5
ER -