Analysis of immunoglobulin levels after exposure of cow's milk protein in mice

This research aimed to analize immunoglobulin levels after exposure of cow's milk protein in mice. We conducted a true experiment with a posttest-only control group method. This experiment used female Balb/C mice and neonate mice (pregnant mice 10 weeks old n=15, neonatal mice 2 weeks old). The sample was divided into low dose group (1 mg CMP per gram weight of mice), high dose group (10 mg CMP per gram weight of mice) and control group that consisted 5 pregnant mice respectively. The cow's milk protein allergens were induced in pregnant mice. We took blood from the pregnant mice, and their fetuses were taken for subclass immunoglobulin examination (Ig A, IgM, IgE, Ig G) by using an ELISA (Enzyme-Linked Immunosorbent Assay) kit. In low dose group we found a significant increase (p<0,05) in IgG-1, IgG-2a, and IgG-2b levels in pregnant mice and IgG-1, IgG-2a, IgG-2b, and IgG-3 in neonate mice which described in table 1. While for the high dose group described in table 2, similar results occurred in IgG-1, IgG-2a, and IgG-3 levels (p<0.05) in pregnant mice and IgG-1, IgG-2a, IgG-2b, and IgG-3 in neonate mice. In high-dose group we also found an increase in IgA, IgM, and IgE (p<0.05) in either the neonate or pregnant mice. However, in low dose group there was only a significant increase in IgM (p=0.004) and IgE (p=0,000) in neonate mice and only IgE (P=0,000) in pregnant mice. In conclusion, in low dose group we found a significant increase in IgG-1, IgG-2a, IgG-2b in both pregnant and neonate mice, IgG-3 and IgE in pregnant mice, IgM and IgE in neonate mice. While for the high dose group similar results occurred in IgG-1, IgG-2a, IgG-3, IgA, IgM, and IgE levels in both pregnant and neonate mice and IgG-2b in neonate mice.