TY - JOUR
T1 - Analysis of human decomposition effect on DNA quality with short tandem repeats [STRS] combined index DNA system [CODIS]
AU - Sulistyorini, Nily
AU - Yudianto, Ahmad
AU - Ula, Ma’Rifatul
AU - Sumino, Renny
AU - Setiawan, Fery
N1 - Funding Information:
The author and the co- authors acknowledges to Rector of Universitas Airlangga that has given the funding to the program which called Penelitian Unggulan Fakultas (PUF) Universitas Airlangga held in 2019. The author would like to thank the Rector of Universitas Airlangga which has given the funding from the program called Penelitian Unggulan Fakultas Universitas Airlangg in 2019.
Funding Information:
This study has been agreed by ethical commite which number 33/hrecc.fodm/IV/2019 Acknowledgement: The author would like to thank the Rector of Universitas Airlangga which has given the funding from the program called Penelitian Unggulan Fakultas Universitas Airlangg in 2019.
Publisher Copyright:
© 2020, Institute of Medico-Legal Publications. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Introduction: Personal identification is a problem in criminal or civil cases. Exact determination of personal identity is very important in investigation as any error can be fatal in the judicial process. The process of identification that is often used is through DNA analysis. The problem that often becomes a serious problem for both forensic DNA expert and other DNA expert is the condition of degraded DNA. Method and Materials: This study was conducted by analysing DNA damage patterns using CODIS STR (Short Tandem Repeat) markers to effect the decay process. The type of research used by experimental laboratories, with research design used is time series. Results and Discussion: The result of DNA sample extraction from Psoas and Masseter muscle samples shows the average minimum range of DNA levels for DNA typing is 0.25 ng with purity of 1.8 – 2(1). The result of this study prove the effect of decomposition on DNA concentration on each muscle. This study is also showed a decrease in levels and purity in the samples of Psoas and Masseter muscle tissue buried in soil, sea/salt water and river/fresh water from day 1, 7, 14, 20 and 40. Conclusion: Psoas and Masseter muscle can be alternative material for forensic identification. The success of sequence mapping pattern from STR loci are TPOX, THO1, and CS1PO according to each GC content. GC content of THO1 and TPOX has the same relative value of 0,48, compared to CSF1PO value of 0,33.
AB - Introduction: Personal identification is a problem in criminal or civil cases. Exact determination of personal identity is very important in investigation as any error can be fatal in the judicial process. The process of identification that is often used is through DNA analysis. The problem that often becomes a serious problem for both forensic DNA expert and other DNA expert is the condition of degraded DNA. Method and Materials: This study was conducted by analysing DNA damage patterns using CODIS STR (Short Tandem Repeat) markers to effect the decay process. The type of research used by experimental laboratories, with research design used is time series. Results and Discussion: The result of DNA sample extraction from Psoas and Masseter muscle samples shows the average minimum range of DNA levels for DNA typing is 0.25 ng with purity of 1.8 – 2(1). The result of this study prove the effect of decomposition on DNA concentration on each muscle. This study is also showed a decrease in levels and purity in the samples of Psoas and Masseter muscle tissue buried in soil, sea/salt water and river/fresh water from day 1, 7, 14, 20 and 40. Conclusion: Psoas and Masseter muscle can be alternative material for forensic identification. The success of sequence mapping pattern from STR loci are TPOX, THO1, and CS1PO according to each GC content. GC content of THO1 and TPOX has the same relative value of 0,48, compared to CSF1PO value of 0,33.
KW - Decomposed
KW - Identification
KW - STR CODIS
UR - http://www.scopus.com/inward/record.url?scp=85100218291&partnerID=8YFLogxK
U2 - 10.37506/ijfmt.v14i4.12126
DO - 10.37506/ijfmt.v14i4.12126
M3 - Article
AN - SCOPUS:85100218291
SN - 0973-9122
VL - 14
SP - 3245
EP - 3251
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
IS - 4
ER -