TY - JOUR
T1 - Analysis of hepatitis B virus genotype and gene mutation in patients with advanced liver disease in east Kalimantan, Indonesia
AU - Wahyuni, Rury Mega
AU - Utsumi, Takako
AU - Juniastuti,
AU - Yano, Yoshihiko
AU - Murti, Ignatia Sinta
AU - Amin, Mochamad
AU - Yamani, Laura Navika
AU - Istimagfiroh, Anittaqwa
AU - Purwono, Priyo Budi
AU - Soetjipto,
AU - Lusida, Maria Inge
AU - Hayashi, Yoshitake
N1 - Funding Information:
The present study was supported by a Grant-in-Aid from the Ministry of Education, Culture, Sports, Science and Technology, Japan (grant no. 16H05826) and a Grant-in-Aid from the Japan Initiative for Global Research Network on Infectious Disease supported by The Ministry of Education, Culture, Sports, Science and Technology, Japan, and in part by a Grant-in-Aid from Professor Dato' Sri Tahir through Tahir professorship, Indonesia.
Publisher Copyright:
© 2019, Spandidos Publications. All rights reserved.
PY - 2019/5
Y1 - 2019/5
N2 - Liver cirrhosis (LC) and hepatocellular carcinoma (HCC) are life-threatening conditions frequently associated with chronic hepatitis B virus (HBV) infection in Asian countries, including Indonesia. HBV genotypes and several specific mutations are associated with disease progression. To clarify the geographical variation in viral characteristics, HBV genotypes and gene mutations were investigated in patients with advanced liver disease (ALD) in Samarinda, East Kalimantan, Indonesia. Sera were collected from 41 patients with ALD at Abdul Wahab Sjahranie Hospital and HBV carriers from Red Cross Center blood bank in Samarinda, and screened for hepatitis B surface antigen and hepatitis B e-antigen. Liver function data were obtained from the medical records from each patient. HBV genotype and gene mutations were determined by polymerase chain reaction sequencing. Analysis of HBV isolates indicated that genotype B was the most frequent genotype, at 85.4 and 97.8%, followed by C, at 14.6 and 2.2%, in patients with ALD and in HBV carriers, respectively. The C1505A mutation in X region, T1753V and A1762T/G1764A mutations in the basal core promoter region and C1858T in precore (PC) region were frequent and only detected in patients with ALD (28.9, 40, 73.5 and 17.6%, respectively), whereas the G1896A mutation in the PC region was frequently detected in HBV carriers. The presence of HBV genotype B and certain HBV gene mutations were characteristic of patients with ALD in East Kalimantan.
AB - Liver cirrhosis (LC) and hepatocellular carcinoma (HCC) are life-threatening conditions frequently associated with chronic hepatitis B virus (HBV) infection in Asian countries, including Indonesia. HBV genotypes and several specific mutations are associated with disease progression. To clarify the geographical variation in viral characteristics, HBV genotypes and gene mutations were investigated in patients with advanced liver disease (ALD) in Samarinda, East Kalimantan, Indonesia. Sera were collected from 41 patients with ALD at Abdul Wahab Sjahranie Hospital and HBV carriers from Red Cross Center blood bank in Samarinda, and screened for hepatitis B surface antigen and hepatitis B e-antigen. Liver function data were obtained from the medical records from each patient. HBV genotype and gene mutations were determined by polymerase chain reaction sequencing. Analysis of HBV isolates indicated that genotype B was the most frequent genotype, at 85.4 and 97.8%, followed by C, at 14.6 and 2.2%, in patients with ALD and in HBV carriers, respectively. The C1505A mutation in X region, T1753V and A1762T/G1764A mutations in the basal core promoter region and C1858T in precore (PC) region were frequent and only detected in patients with ALD (28.9, 40, 73.5 and 17.6%, respectively), whereas the G1896A mutation in the PC region was frequently detected in HBV carriers. The presence of HBV genotype B and certain HBV gene mutations were characteristic of patients with ALD in East Kalimantan.
KW - Genotype
KW - Hepatitis B virus
KW - Indonesia
KW - Liver disease
KW - Mutation
UR - http://www.scopus.com/inward/record.url?scp=85070250799&partnerID=8YFLogxK
U2 - 10.3892/br.2019.1202
DO - 10.3892/br.2019.1202
M3 - Article
AN - SCOPUS:85070250799
SN - 2049-9434
VL - 10
SP - 303
EP - 310
JO - Biomedical Reports
JF - Biomedical Reports
IS - 5
ER -
