TY - JOUR
T1 - Analysis of gastric microbiota and Helicobacter pylori infection in gastroesophageal reflux disease
AU - Sugihartono, Titong
AU - Fauzia, Kartika Afrida
AU - Miftahussurur, Muhammad
AU - Waskito, Langgeng Agung
AU - Rejeki, Purwo Sri
AU - I’tishom, Reny
AU - Alfaray, Ricky Indra
AU - Doohan, Dalla
AU - Amalia, Rizki
AU - Savitri, Camilia Metadea Aji
AU - Rezkitha, Yudith Annisa Ayu
AU - Akada, Junko
AU - Matsumoto, Takashi
AU - Yamaoka, Yoshio
N1 - Funding Information:
This study was supported in part by grants from the (DK62813) (YY) and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan (221S0002, 16H06279, 18KK0266, 19H03473) (YY) and 18K16182 (TM), 17K09353(JA). This work was also supported by the Japan Society for the Promotion of Science Institutional Program for Young Researcher Overseas Visits and the Strategic Funds for the Promotion of Science and Technology Agency (JST) for YY. This study was also supported by Thailand Science Research and Innovation Fundamental Fund, Bualuang ASEAN Chair Professorship at Thammasat University, and Center of Excellence in Digestive Diseases, Thammasat University, Thailand for YY. Ricky Indra Alfaray, Rizki Amalia and Camilia Metadea is the MEXT student for 2019 (RIA) and 2021 (RA, CM). This study also supported by the World Class Research 2020 Grant of Directorate of Research and Community Service, Deputy for Strengthening Research and Development Ministry of Research and Technology/ National Research and Innovation Agency, Indonesia (MM).
Publisher Copyright:
© 2022, The Author(s).
PY - 2022/12
Y1 - 2022/12
N2 - Background: We evaluated the microbiota in the stomach of Gastroesophageal Reflux Disease (GERD) patients. We compared Erosive Reflux Disease (ERD) to gastritis and Non-erosive Reflux Disease (NERD) subjects by 16S rRNA approach on gastric biopsy specimens. A total of 197 subjects were included consisting of gastritis (68; 34.52%), ERD (55; 27.92%), and NERD (74; 37.56%). After quality filtering, 187 samples were included for OTU analysis using Qiime2. Results: We observed a significant difference in alpha diversity (Shannon and Simpson indexes were P = 0.0016 and P = 0.017, respectively). A significant decrease in alpha diversity index was observed in NERD with Helicobacter pylori (H. pylori)-positive subjects than in gastritis (Simpson index P = 0.022; Shannon index P = 0.029), indicating a significant influence of H. pylori on the diversity in the stomach despite the diseases. In H. pylori-negative samples, alpha diversity measurement by the abundance coverage estimates (ACE) and Fisher Test revealed that ERD had significantly lower richness than gastritis and NERD groups (P = 0.00012 and P = 0.00043, respectively). Anaerobacillus sp. could only be found in ERD patients by LEFse analysis. Conclusions: The presence of ERD could alter microbiome diversity. A negative correlation between H. pylori and ERD is shown in this microbiome study but not in NERD.
AB - Background: We evaluated the microbiota in the stomach of Gastroesophageal Reflux Disease (GERD) patients. We compared Erosive Reflux Disease (ERD) to gastritis and Non-erosive Reflux Disease (NERD) subjects by 16S rRNA approach on gastric biopsy specimens. A total of 197 subjects were included consisting of gastritis (68; 34.52%), ERD (55; 27.92%), and NERD (74; 37.56%). After quality filtering, 187 samples were included for OTU analysis using Qiime2. Results: We observed a significant difference in alpha diversity (Shannon and Simpson indexes were P = 0.0016 and P = 0.017, respectively). A significant decrease in alpha diversity index was observed in NERD with Helicobacter pylori (H. pylori)-positive subjects than in gastritis (Simpson index P = 0.022; Shannon index P = 0.029), indicating a significant influence of H. pylori on the diversity in the stomach despite the diseases. In H. pylori-negative samples, alpha diversity measurement by the abundance coverage estimates (ACE) and Fisher Test revealed that ERD had significantly lower richness than gastritis and NERD groups (P = 0.00012 and P = 0.00043, respectively). Anaerobacillus sp. could only be found in ERD patients by LEFse analysis. Conclusions: The presence of ERD could alter microbiome diversity. A negative correlation between H. pylori and ERD is shown in this microbiome study but not in NERD.
KW - ERD
KW - GERD
KW - Helicobacter pylori
KW - Infectious disease
KW - Microbiome
KW - NERD
UR - http://www.scopus.com/inward/record.url?scp=85138780622&partnerID=8YFLogxK
U2 - 10.1186/s13099-022-00510-3
DO - 10.1186/s13099-022-00510-3
M3 - Article
AN - SCOPUS:85138780622
VL - 14
JO - Gut Pathogens
JF - Gut Pathogens
SN - 1757-4749
IS - 1
M1 - 38
ER -
