TY - JOUR
T1 - An effective concentration of propolis extract to inhibit the activity of Streptococcus mutans glucosyltransferase enzyme
AU - Marsetyo, Riyan Iman
AU - Andyningtyas, Sagita Putri
AU - Zamrutizahra, Chonny Salsabilla
AU - Fadela, Ivan Nur
AU - Subiwahjudi, Agus
AU - Widjiastuti, Ira
N1 - Publisher Copyright:
© Dental Journal 2021.
PY - 2021/12
Y1 - 2021/12
N2 - Background: According to Riset Kesehatan Dasar (Riskesdas) (2013) and the World Health Organisation (WHO), caries is still a global problem and highly prevalent in Indonesia. Caries is mainly caused by Streptococcus mutans with virulence factors known as glucosyltransferase (GTF). The GTF enzyme contribute to the pathogenesis of caries by converting sucrose to fructose and glucan, which are then used in the formation of biofilms and dental plaques. Natural propolis compounds containing flavonoids, terpenoids, saponins and tannins, can inhibit GTF enzyme activity. Purpose: This study aimed to determine an effective concentration of propolis extract for inhibiting the S. mutans GTF enzyme activity. Methods: This study used propolis extract at 14 μg/ mL, 16 μg/m and 1 μg/ mL to determine the inhibitory effect on S. mutans GTF enzyme activity. The GTF enzyme were obtained from the supernatant from S. mutans culture centrifugation. The GTF enzyme activity was measured using high-performance liquid chromatography (HPLC) to calculate the fructose level. Results: The mean fructose concentration at 14 μg/mL, 16 μg/mL, and 18μg/mL were 3.31%, 1.56%, and 0.29%, respectively. Conclusion: The most effective concentration of propolis extract for inhibiting the effect of S. mutans GTF enzyme activity is 14 μg/mL.
AB - Background: According to Riset Kesehatan Dasar (Riskesdas) (2013) and the World Health Organisation (WHO), caries is still a global problem and highly prevalent in Indonesia. Caries is mainly caused by Streptococcus mutans with virulence factors known as glucosyltransferase (GTF). The GTF enzyme contribute to the pathogenesis of caries by converting sucrose to fructose and glucan, which are then used in the formation of biofilms and dental plaques. Natural propolis compounds containing flavonoids, terpenoids, saponins and tannins, can inhibit GTF enzyme activity. Purpose: This study aimed to determine an effective concentration of propolis extract for inhibiting the S. mutans GTF enzyme activity. Methods: This study used propolis extract at 14 μg/ mL, 16 μg/m and 1 μg/ mL to determine the inhibitory effect on S. mutans GTF enzyme activity. The GTF enzyme were obtained from the supernatant from S. mutans culture centrifugation. The GTF enzyme activity was measured using high-performance liquid chromatography (HPLC) to calculate the fructose level. Results: The mean fructose concentration at 14 μg/mL, 16 μg/mL, and 18μg/mL were 3.31%, 1.56%, and 0.29%, respectively. Conclusion: The most effective concentration of propolis extract for inhibiting the effect of S. mutans GTF enzyme activity is 14 μg/mL.
KW - Glucosyltransferase enzyme
KW - Streptococcus mutans
KW - medicine
KW - propolis extract
UR - http://www.scopus.com/inward/record.url?scp=85147485733&partnerID=8YFLogxK
U2 - 10.20473/J.DJMKG.V54.I4.P186-189
DO - 10.20473/J.DJMKG.V54.I4.P186-189
M3 - Article
AN - SCOPUS:85147485733
SN - 1978-3728
VL - 54
SP - 186
EP - 189
JO - Dental Journal
JF - Dental Journal
IS - 4
ER -