Amnion membrane-derived mesenchymal stem cells (MSCs) migration and homing in a burn-wounded mouse model as tracked using ivis imaging

Fedik A. Rantam; Abrahamm Ahmad Ali Firdaus; Evi Awwalia; Anak Agung Ayu Meidiary; Purwati Purwati; Eryk Hendrianto; Igo Syaiful Ihsan; Helen Susilowati

doi:10.35124/bca.2019.19.S2.4803

Amnion membrane-derived mesenchymal stem cells (MSCs) migration and homing in a burn-wounded mouse model as tracked using ivis imaging

Fedik A. Rantam, Abrahamm Ahmad Ali Firdaus, Evi Awwalia, Anak Agung Ayu Meidiary, Purwati Purwati, Eryk Hendrianto, Igo Syaiful Ihsan, Helen Susilowati

Research output: Contribution to journal › Article › peer-review

Abstract

Mesenchymal stem cells (MSCs) are undifferentiated cells with potential for therapeutic use in degenerative disease, trauma, or injury. In this study, wounds on the dorsal skin of mice resulting from an iron scaffold were injected with 105 activated MSCs. Crude secretions obtained from maintenance stem cell medium was labeled using green fluorescent protein following an intravenous lateral tail administration. Bioluminescent imaging to determine the MSC migration into the cutaneous burn injury site was performed after 0, 24 and 48 hours. The results obtained suggest that active MSCs accelerate the burn healing process.

Original language	English
Pages (from-to)	4803-4806
Number of pages	4
Journal	Biochemical and Cellular Archives
DOIs	https://doi.org/10.35124/bca.2019.19.S2.4803
Publication status	Published - 1 Dec 2019

Keywords

Bioluminescent imaging
Burn injury
Crude secretions
MSC migration

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10.35124/bca.2019.19.S2.4803

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title = "Amnion membrane-derived mesenchymal stem cells (MSCs) migration and homing in a burn-wounded mouse model as tracked using ivis imaging",

abstract = "Mesenchymal stem cells (MSCs) are undifferentiated cells with potential for therapeutic use in degenerative disease, trauma, or injury. In this study, wounds on the dorsal skin of mice resulting from an iron scaffold were injected with 105 activated MSCs. Crude secretions obtained from maintenance stem cell medium was labeled using green fluorescent protein following an intravenous lateral tail administration. Bioluminescent imaging to determine the MSC migration into the cutaneous burn injury site was performed after 0, 24 and 48 hours. The results obtained suggest that active MSCs accelerate the burn healing process.",

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doi = "10.35124/bca.2019.19.S2.4803",

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AU - Rantam, Fedik A.

AU - Firdaus, Abrahamm Ahmad Ali

AU - Awwalia, Evi

AU - Meidiary, Anak Agung Ayu

AU - Purwati, Purwati

AU - Hendrianto, Eryk

AU - Ihsan, Igo Syaiful

AU - Susilowati, Helen

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Mesenchymal stem cells (MSCs) are undifferentiated cells with potential for therapeutic use in degenerative disease, trauma, or injury. In this study, wounds on the dorsal skin of mice resulting from an iron scaffold were injected with 105 activated MSCs. Crude secretions obtained from maintenance stem cell medium was labeled using green fluorescent protein following an intravenous lateral tail administration. Bioluminescent imaging to determine the MSC migration into the cutaneous burn injury site was performed after 0, 24 and 48 hours. The results obtained suggest that active MSCs accelerate the burn healing process.

AB - Mesenchymal stem cells (MSCs) are undifferentiated cells with potential for therapeutic use in degenerative disease, trauma, or injury. In this study, wounds on the dorsal skin of mice resulting from an iron scaffold were injected with 105 activated MSCs. Crude secretions obtained from maintenance stem cell medium was labeled using green fluorescent protein following an intravenous lateral tail administration. Bioluminescent imaging to determine the MSC migration into the cutaneous burn injury site was performed after 0, 24 and 48 hours. The results obtained suggest that active MSCs accelerate the burn healing process.

KW - Bioluminescent imaging

KW - Burn injury

KW - Crude secretions

KW - MSC migration

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JO - Biochemical and Cellular Archives

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ER -

Amnion membrane-derived mesenchymal stem cells (MSCs) migration and homing in a burn-wounded mouse model as tracked using ivis imaging

Abstract

Keywords

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