Alteplase in preventing posterior capsule opacity by plasminogen activator inhibitor-1 and type i collagen expression inhibition in a posterior capsule opacity model with fibrin reaction in vitro: A experimental laboratory study

Rina Wulandari, Indri Wahyuni, Arifa Mustika, Windhu Purnomo, Nurwasis

Research output: Contribution to journalArticlepeer-review

Abstract

To investigate the effect of alteplase on plasminogen activator inhibitor -1 (PAI-1) and type I collagen expression in a posterior capsule opacity (PCO) model with fibrin reaction in vitro. Cultured human lens epithelial cells (HLEC) were isolated from the anterior lens capsule following a capsulotomy during cataract surgery. Cultured HLEC went into an in vitro wound scratch assay on fibrin coated dishes and were then divided into 4 groups consisting of a FBS 10% control group, and treatment groups of alteplase 25, 50 and 100 μg/ml. The effect of alteplase on plasminogen activator inhibitor (PAI-1) and type I collagen expression in PCO and its reaction with fibrin was investigated, determined by fluorescein intensity, labelled with fluorescein isothiocyanate (FITC) antibody of PAI-1 and type I collagen. The difference between expression levels among the groups was analyzed using a Kolmogorov-Smirnov test followed by a posthoc test showing a significance level of p<0.05. The lowest PAI-1 expression was obtained in the group treated with alteplase 100 ìg /ml (1.26 × 106± 3.31 × 105) compared to the control group (6.27 × 106± 2.37 × 105 , p= 0.00); a significant result was also obtained among treatment groups, and showed that inhibition behaves in a dose dependent way. All treatment groups significantly inhibited type I collagen as compared to control group (3.54 × 106 ± 8.28 × 105, p= 0.00), but the result is not significantly different among treatment groups. The inhibition effect of PAI-1 and type I collagen were influenced from the affects of several another pathways in type I collagen synthesis other than PAI-1. Alteplase showed an inhibition effect on PAI-1 and type I collagen with fibrin reaction in vitro in anterior lens capsule fibrosis, despite the inhibition effect on type I collagen not aligning with the inhibition of PAI-1.

Original languageEnglish
Pages (from-to)4695-4701
Number of pages7
JournalBiochemical and Cellular Archives
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Alteplase
  • Fibrosis
  • HLEC
  • Lens epithelial cell
  • PAI-1
  • PCO
  • Type I collagen

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