TY - JOUR
T1 - Alpha-Glucosidase inhibitory effect of psychotria malayana Jack Leaf
T2 - A rapid analysis using infrared fingerprinting
AU - Nipun, Tanzina Sharmin
AU - Khatib, Alfi
AU - Ahmed, Qamar Uddin
AU - Redzwan, Irna Elina
AU - Ibrahim, Zalikha
AU - Khan, Al'aina Yuhainis Firus
AU - Primaharinastiti, Riesta
AU - Khalifa, Shaden A.M.
AU - El-Seedi, Hesham R.
N1 - Funding Information:
Acknowledgments: T.S.N. would like to thank the Bangabandhu Science and Technology Fellowship Trust, Ministry of Science and Technology of Bangladesh for the granting of her Ph.D. fellowship. A. Khatib acknowledges the Malaysian Ministry of Education for the Fundamental Research Grant Scheme [FRGS-19-0880697]; S.A.M.K. and H.R.E.-S. are very grateful to the Swedish Research Links Grant VR 2016-05885 and the Department of Molecular Biosciences, Wenner-Grens Institute, Stockholm University, Sweden, for financial support.
Funding Information:
Funding: This work was supported by the Malaysian Ministry of Education for the Fundamental Research Grant Scheme [FRGS-19-0880697] and the Swedish Research Council Vetenskapsrådet (VR Grant 2016–05885).
Funding Information:
This work was supported by the Malaysian Ministry of Education for the Fundamental Research Grant Scheme [FRGS-19-0880697] and the Swedish Research Council Vetenskapsr?det (VR Grant 2016?05885). T.S.N. would like to thank the Bangabandhu Science and Technology Fellowship Trust, Ministry of Science and Technology of Bangladesh for the granting of her Ph.D. fellowship. A. Khatib acknowledges the Malaysian Ministry of Education for the Fundamental Research Grant Scheme [FRGS-19-0880697]; S.A.M.K. and H.R.E.-S. are very grateful to the Swedish Research Links Grant VR 2016-05885 and the Department of Molecular Biosciences, Wenner-Grens Institute, Stockholm University, Sweden, for financial support.
Publisher Copyright:
© 2020 by the authors.
PY - 2020/9
Y1 - 2020/9
N2 - The plant Psychotria malayana Jack belongs to the Rubiaceae family and is known in Malaysia as “meroyan sakat/salung”. A rapid analytical technique to facilitate the evaluation of the P. malayana leaves’ quality has not been well-established yet. This work aimed therefore to develop a validated analytical technique in order to predict the alpha-glucosidase inhibitory action (AGI) of P. malayana leaves, applying a Fourier Transform Infrared Spectroscopy (FTIR) fingerprint and utilizing an orthogonal partial least square (OPLS). The dried leaf extracts were prepared by sonication of different ratios of methanol-water solvent (0, 25, 50, 75, and 100% v/v) prior to the assessment of alpha-glucosidase inhibition (AGI) and the following infrared spectroscopy. The correlation between the biological activity and the spectral data was evaluated using multivariate data analysis (MVDA). The 100% methanol extract possessed the highest inhibitory activity against the alpha-glucosidase (IC50 2.83 ± 0.32 µg/mL). Different bioactive functional groups, including hydroxyl (O-H), alkenyl (C=C), methylene (C-H), carbonyl (C=O), and secondary amine (N-H) groups, were detected by the multivariate analysis. These functional groups actively induced the alpha-glucosidase inhibition effect. This finding demonstrated the spectrum profile of the FTIR for the natural herb P. malayana Jack, further confirming its medicinal value. The developed validated model can be used to predict the AGI of P. malayana, which will be useful as a tool in the plant’s quality control.
AB - The plant Psychotria malayana Jack belongs to the Rubiaceae family and is known in Malaysia as “meroyan sakat/salung”. A rapid analytical technique to facilitate the evaluation of the P. malayana leaves’ quality has not been well-established yet. This work aimed therefore to develop a validated analytical technique in order to predict the alpha-glucosidase inhibitory action (AGI) of P. malayana leaves, applying a Fourier Transform Infrared Spectroscopy (FTIR) fingerprint and utilizing an orthogonal partial least square (OPLS). The dried leaf extracts were prepared by sonication of different ratios of methanol-water solvent (0, 25, 50, 75, and 100% v/v) prior to the assessment of alpha-glucosidase inhibition (AGI) and the following infrared spectroscopy. The correlation between the biological activity and the spectral data was evaluated using multivariate data analysis (MVDA). The 100% methanol extract possessed the highest inhibitory activity against the alpha-glucosidase (IC50 2.83 ± 0.32 µg/mL). Different bioactive functional groups, including hydroxyl (O-H), alkenyl (C=C), methylene (C-H), carbonyl (C=O), and secondary amine (N-H) groups, were detected by the multivariate analysis. These functional groups actively induced the alpha-glucosidase inhibition effect. This finding demonstrated the spectrum profile of the FTIR for the natural herb P. malayana Jack, further confirming its medicinal value. The developed validated model can be used to predict the AGI of P. malayana, which will be useful as a tool in the plant’s quality control.
KW - Fingerprint
KW - Infrared spectroscopy analysis
KW - Orthogonal partial least square
KW - Psychotria malayana
KW - α-glucosidase inhibition
UR - http://www.scopus.com/inward/record.url?scp=85090850444&partnerID=8YFLogxK
U2 - 10.3390/molecules25184161
DO - 10.3390/molecules25184161
M3 - Article
C2 - 32932994
AN - SCOPUS:85090850444
SN - 1420-3049
VL - 25
JO - Molecules
JF - Molecules
IS - 18
M1 - 4161
ER -
