ALP (Alkaline Phosphatase) expression in simple fracture incident in rat (Rattus Norvegicus) femur bone supplemented by apis mellifera honey

Abdullah Hasib, Dian Agustin Wahjuningrum, Muhammad Huda Ramadhan Ibrahim, Hendy Jaya Kurniawan, Rizky Ernawati, Maria Elisea Kiswantoro Hadinoto, Latief Mooduto

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The aim of the experiment was to observe the serum alkaline phosphatase (ALP) expression of Apis mellifera honey supplementation on simple fracture femur bone. Twelve adult male rats were divided into 4 groups. Those groups are control group, which supplemented with aquadest for 1,5 ml and 3 groups were supplemented with 1,5 ml honey solution (1g/Kg BW, 2g/Kg BW, 3g/Kg BW) for 2 weeks. Fracture incident was made at the day before supplementation by osteotomy technique using dental bur in the metaphyseal region of femur reaching inside the bone marrow. At the fifteenth day, serum was obtained by centrifuging the blood than assessed using prestige analyzer 24i based on IFCC method. The data were analyzed using ANOVA and Tukey with SPSS for windows. The result showed a very significantly different (P<0. 01) between honey groups and control group and there was no significant different (P>0. 05) between honey groups. The indication of raising ALP revealed that honey might impact osteoblast. The result indicates that gradual dosage on simple fracture might not affect seriously as honey was organic matter containing bioactive compound as minor constituent not in the form of highly intensive extract. Thus, Apis mellifera honey might enhance ALP serum concentration in the incident of simple fracture.

Original languageEnglish
Pages (from-to)887-891
Number of pages5
JournalJournal of International Dental and Medical Research
Volume13
Issue number3
Publication statusPublished - 2020

Keywords

  • ALP
  • Bone
  • Honey

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