ALP (Alkaline Phosphatase) expression in simple fracture incident in rat (Rattus Norvegicus) femur bone supplemented by apis mellifera honey

The aim of the experiment was to observe the serum alkaline phosphatase (ALP) expression of Apis mellifera honey supplementation on simple fracture femur bone. Twelve adult male rats were divided into 4 groups. Those groups are control group, which supplemented with aquadest for 1,5 ml and 3 groups were supplemented with 1,5 ml honey solution (1g/Kg BW, 2g/Kg BW, 3g/Kg BW) for 2 weeks. Fracture incident was made at the day before supplementation by osteotomy technique using dental bur in the metaphyseal region of femur reaching inside the bone marrow. At the fifteenth day, serum was obtained by centrifuging the blood than assessed using prestige analyzer 24i based on IFCC method. The data were analyzed using ANOVA and Tukey with SPSS for windows. The result showed a very significantly different (P<0. 01) between honey groups and control group and there was no significant different (P>0. 05) between honey groups. The indication of raising ALP revealed that honey might impact osteoblast. The result indicates that gradual dosage on simple fracture might not affect seriously as honey was organic matter containing bioactive compound as minor constituent not in the form of highly intensive extract. Thus, Apis mellifera honey might enhance ALP serum concentration in the incident of simple fracture.