Alkaloid fraction of jarong (achyranthes aspera linn) leaf induced apoptosis breast cancer cell through p53 pathways

Achyranthesasperalinn leaf is used to treat cancer, particularly breast and cervix cancer. The alkaloid fraction of leaves Achyranthesasperalinn cause of death in accordance with the mechanism of myeloma cell apoptosis and necrosis associated with p53 gene and cycline dependent kinase 4.We employed mice with mammary cancer from benzo(α)pyeren induction to elucidate activity of leave extract of Achyranthesasperalinn to cure mammary cancer. The Mice with mammary cancer were divided into 5 groups there are group were treated with 0 mg/kg, 10 mg / kg, 30 mg / kg, and 100 mg / kg alkaloid Achyranthesaspera Linn by oraleveryday respectively; and group were treated with a standard anticancer drug cyclophosphamide 6 mg / kg for positive control. We also used healthy mice were treated with oilumolifarum as negative control. After 8 weeks treatment, mice were sacrificed to evaluate p53 and CDK expression level in mammary tissue. The results show that Achyranthesaspera Linn alkaloidsincreasedapoptosis in breast cancer cell through upregulate p53expression and inhibit CDK level in mammary cancer cells. Moreover, Achyranthesasperalinnin dose 10 mg/kgbw until 100 mg/kgbwshown have similar effect with Cyclophosamide 6 mg/kg bw.