Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells

Suryo Kuncorojakti, Watchareewan Rodprasert, Supansa Yodmuang, Thanaphum Osathanon, Prasit Pavasant, Sayamon Srisuwatanasagul, Chenphop Sawangmake

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Background: Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp-derived stem cell (hDPSC)-derived IPCs by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. Results: The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG and ALGPA-encapsulated hDPSC-derived IPCs maintained viability for at least 336 h and sustained pancreatic endoderm marker (NGN3), pancreatic islet markers (NKX6.1, MAF-A, ISL-1, GLUT-2 and INSULIN), and intracellular pro-insulin and insulin expressions for at least 14 days. Functional analysis revealed a glucose-responsive C-peptide secretion of ALG- and ALGPA-encapsulated hDPSC-derived IPCs at 14 days post-encapsulation. Conclusion: ALG and ALGPA encapsulations efficiently preserved the viability and functionality of hDPSC-derived IPCs in vitro and could be the potential transplantation platform for further clinical application.

Original languageEnglish
Article number23
JournalJournal of Biological Engineering
Volume14
Issue number1
DOIs
Publication statusPublished - 24 Aug 2020
Externally publishedYes

Keywords

  • Alginate
  • Dental pulp stem cells (DPSCs)
  • Diabetes mellitus
  • Encapsulation
  • Insulin-producing cells (IPCs)
  • Pluronic F127

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