AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells

Gaku Okui; Kei Tobiume; Andra Rizqiawan; Kazuhiro Yamamoto; Hideo Shigeishi; Shigehiro Ono; Koichiro Higashikawa; Nobuyuki Kamata

doi:10.1002/jcb.24545

AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells

Gaku Okui, Kei Tobiume, Andra Rizqiawan, Kazuhiro Yamamoto, Hideo Shigeishi, Shigehiro Ono, Koichiro Higashikawa, Nobuyuki Kamata

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

In this study, we found that wounding of a confluent monolayer of squamous cell carcinoma (SCC) cells induced epithelial-mesenchymal transition (EMT) specifically at the edge of the wound. This process required the combined stimulation of TGFβ, TNFα, and PDGF-D. Such a combined cytokine treatment of confluent monolayers of the cells upregulated the expression levels of Snail and Slug via PI3K. The PI3K downstream effector, AKT, was dispensable for the upregulation of Snail and Slug, but essential for enabling EMT in response to upregulation of Snail and Slug.

Original language	English
Pages (from-to)	2039-2049
Number of pages	11
Journal	Journal of Cellular Biochemistry
Volume	114
Issue number	9
DOIs	https://doi.org/10.1002/jcb.24545
Publication status	Published - Sept 2013
Externally published	Yes

Keywords

AKT
EMT
PI3K
SNAIL
SQUAMOUS CELL CARCINOMA

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10.1002/jcb.24545

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title = "AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells",

abstract = "In this study, we found that wounding of a confluent monolayer of squamous cell carcinoma (SCC) cells induced epithelial-mesenchymal transition (EMT) specifically at the edge of the wound. This process required the combined stimulation of TGFβ, TNFα, and PDGF-D. Such a combined cytokine treatment of confluent monolayers of the cells upregulated the expression levels of Snail and Slug via PI3K. The PI3K downstream effector, AKT, was dispensable for the upregulation of Snail and Slug, but essential for enabling EMT in response to upregulation of Snail and Slug.",

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T1 - AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells

AU - Okui, Gaku

AU - Tobiume, Kei

AU - Rizqiawan, Andra

AU - Yamamoto, Kazuhiro

AU - Shigeishi, Hideo

AU - Ono, Shigehiro

AU - Higashikawa, Koichiro

AU - Kamata, Nobuyuki

PY - 2013/9

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AB - In this study, we found that wounding of a confluent monolayer of squamous cell carcinoma (SCC) cells induced epithelial-mesenchymal transition (EMT) specifically at the edge of the wound. This process required the combined stimulation of TGFβ, TNFα, and PDGF-D. Such a combined cytokine treatment of confluent monolayers of the cells upregulated the expression levels of Snail and Slug via PI3K. The PI3K downstream effector, AKT, was dispensable for the upregulation of Snail and Slug, but essential for enabling EMT in response to upregulation of Snail and Slug.

KW - AKT

KW - EMT

KW - PI3K

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KW - SQUAMOUS CELL CARCINOMA

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JO - Journal of Cellular Biochemistry

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AKT primes snail-induced EMT concomitantly with the collective migration of squamous cell carcinoma cells

Abstract

Keywords

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