TY - JOUR
T1 - Adverse effects of mercury exposure in ddw strain mice during organogenesis
AU - Darmanto, Win
AU - Husen, Saikhu Akhmad
AU - Hasan, Arif Abu
AU - Susilo, Raden Joko Kuncoroningrat
AU - Hayaza, Suhailah
AU - Punnapayak, Hunsa
N1 - Publisher Copyright:
© 2020, Institute of Medico-Legal Publications. All rights reserved.
PY - 2020/10/1
Y1 - 2020/10/1
N2 - Mercury (Hg) was known as a teratogenic which is distributed in tissue. This study aims to determine the retention and embryotoxicity of Hg-exposed pregnant mice. Thirty female mice was treated with HgCl2 (mercuric chloride). HgCl2 (5 and 6 kg/mg BW) was inducted in pregnant mice at 9 and 11 gestational days. Hg levels were measured in hair, uterus, liver, kidney, brain, blood, placenta, visceral fetus, and fetus brain on the 18th day of gestation using the atomic absorption spectrophotometry (AAS) method. Embryotoxicity test on the fetus was carried out after the surgery took place. Scales and calipers are used to calculate fetal weight and crown rump length. Statistical tests were analyzed using the SPSS 21 program. The results showed that the liver, kidney, brain, visceral fetal, and fetal brain were significantly increased (P < 0.05) in the treatment group at pregnant mice. Hg also produced a significant difference (P < 0.05) on the decrease in live fetuses, fetuses, body weight, and crown rump length and an increase in resorbed fetuses. Hg accumulation in the body apparently can cause adverse effects in pregnant mice.
AB - Mercury (Hg) was known as a teratogenic which is distributed in tissue. This study aims to determine the retention and embryotoxicity of Hg-exposed pregnant mice. Thirty female mice was treated with HgCl2 (mercuric chloride). HgCl2 (5 and 6 kg/mg BW) was inducted in pregnant mice at 9 and 11 gestational days. Hg levels were measured in hair, uterus, liver, kidney, brain, blood, placenta, visceral fetus, and fetus brain on the 18th day of gestation using the atomic absorption spectrophotometry (AAS) method. Embryotoxicity test on the fetus was carried out after the surgery took place. Scales and calipers are used to calculate fetal weight and crown rump length. Statistical tests were analyzed using the SPSS 21 program. The results showed that the liver, kidney, brain, visceral fetal, and fetal brain were significantly increased (P < 0.05) in the treatment group at pregnant mice. Hg also produced a significant difference (P < 0.05) on the decrease in live fetuses, fetuses, body weight, and crown rump length and an increase in resorbed fetuses. Hg accumulation in the body apparently can cause adverse effects in pregnant mice.
KW - Fetus
KW - Mercury
KW - Mice
KW - Organogenesis
KW - Retention
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=85099485615&partnerID=8YFLogxK
U2 - 10.37506/ijfmt.v14i4.12277
DO - 10.37506/ijfmt.v14i4.12277
M3 - Article
AN - SCOPUS:85099485615
SN - 0973-9122
VL - 14
SP - 4058
EP - 4064
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
IS - 4
ER -