Advance glycation end products induced an altetration of cell proliferation in human umbilical cord mesenchymal stem cells

Diabetes and its complications quickly become the most significant cause of world morbidity and mortality. Increased glucose levels begin to form an Advanced glycation end products (AGEs) that lead to cell activity alteration. This study was carried out to evaluate the viability of hUCMSCs cells against AGE-BSA at various concentrations. This study was an in vitro laboratory experimental research. hUCMSCs used in this study were the results of thawing isolates of hUCMSCs cells and were prepared on the fourth passage. hUCMSCs were seeded in 96 well alpha MEM medium and various concentrations of AGE-BSA 25 mg/ml, 12.5 mg/ml, 6.25 mg/ml, 3.125 mg/ml, 1.56 mg/ml, 0.78 mg/ml and 0.39 mg/ml. After 3 days, the MTT assay was carried out to know the viability test of hUCMSCs induced by AGE-BSA. The data obtained was then collected and analyzed using ANOVA to determine differences between groups in the AGE-BSA viability test.The highest cell living percentage were obtained in the AGE-BSA concentration group 0.39 mg/ml and the lowest in the 25 mg/ml concentration group. In the AGEBSA group with a concentration of 0.39 mg/ml obtained the number of living cells was 72.50 ± 1.69% while at a concentration of 25 mg/ml the percentage of living cells was 66.43 ± 5.43%. There were no significant different for each group (p < 0.05). AGE-BSA affect cell proliferation activity based on the results of the percentage of cells that live on hUCMSCs that have been exposed to AGE-BSA.