Blood purification is essential in the medical field to separate and eliminate toxins and impurities in the bloodstream. To eliminate all the toxic substances in the body, the hemodialysis membrane must have adsorption properties to adsorb all the toxins that cannot be removed by diffusion through the membrane pore. A hemodialysis membrane of chitosan starch without crosslinking and crosslinking has been made to separate creatinine compounds. Chitosan-starch crosslink membranes are made using glutaraldehyde as a crosslinking agent. Chitosan-starch membrane without crosslinking has a tensile strength of l0.07 kgf/mm2, while the membrane of crosslinked chitosan has a better tensile strength of 66.79 kgf/mm2. The chitosan-starch membrane without crosslinking has clean water fluxes of 9l, 60 L/m2 hour, while the chitosan-starch membrane crosslinking has water fluxes of 89.l7 L/m2 hour. Chitosan-starch membrane without crosslinking can remove creatinine of 6.427 mg Crt/gram membrane where the amount removed by adsorption reaches 3.828 mg Crt/g membrane. Chitosan-starch crosslinked membrane can remove creatinine of 5,682 mg Crtgram membrane with the amount removed by adsorption reaching 3.589 mgCrt/gram membrane. Chitosan-starch hemodialysis membranes can remove creatinine through a combination of adsorption and diffusion in one step.