Adenosine Deaminase (ADA) Levels in Suspected Tuberculosis Patients with Results of Interferon-Gamma Results Release Assay (IGRA) Positive and Negative

Introduction: Tuberculosis is one of the major public health problems and remains a global health issues including Indonesia, which is caused by the bacterium Mycobacterium tuberculosis (MTb). Adenosine deaminase (ADA) is an enzyme in purin catabolism process which catalyze ademosine into inosine and deoksiadenosine into deoksiinosine and plays an important role in lymphoid cell differentiation. ADA is elevated in TB pleural. Aim of this study was to determine ADA levels in suspected TB patients. Materials and Methods: This study was a cross sectional analytic observational. The subjects were 60 samples, 30 samples with IGRA positive and 30 samples with IGRA negative. The participants’s age range from 18 to 90 years old. The examination of IGRA by ELISA method, ADA by enzymatic colorimetry method. This study was conducted from January to April 2020 at Gleneagles Diagnostic Centre Laboratory in Surabaya. Results: There was a significant difference of ADA levels in IGRA positive and negative (p< 0.05), which means the ADA value of TB patients are higher than non TB patients. Conclusion: As there is still needed further research to determine the benefits of ADA relate to other examinations, to interprete ADA levels and IGRA in TB cases should be compared to clinical symptoms and radiology reports.