Acute toxicity test of liquid smoke of rice hull (oryza sativa) on mice (mus musculus)

Liquid smoke as product of rice hull's pyrolysis is contained high phenolic compound such as phenol and guaiacol. These phenolic coumpound have been shown to have anti-inflammatory and antioxidant properties. Because of these properties, liquid smoke rice hull has the potential to be developed as a medicine. However, the safety of liquid smoke rice hull must be ensured to be used as a medicine. The purpose of this study is to analyzed the effects of acute toxicity of liquid smoke rice hull sativa) on mice (Mus musculus). Laboratory experiment using 25 experimental mice which divided into 5 groups: control group (given aquadest), group 1, 2, 3 and 4 (given liquid smoke with a single dose by 50, 500, 5000, and 15000 mg/kg body weight orally). Symptoms of toxicity, weight changes, and the number of dead animals were noted for 7 days, whereas liver histopathology on the dead and living objects were observed after the observation period ended. The LD50 of liquid smoke rice hull >15000mg/kg body weight. No toxicity symptoms were found in living animals. Changes in body weight in all groups tended to increase. Histopathology of the liver did not show a significant difference between the control group and the liquid smoke group (p=0.26). Liquid smoke of rice hull (Oryza sativa) does not have an acute toxicity effect on mice (Mus musculus).