Activity test of anti-stress from extract of Datura metel seeds with ethanol solvent towards blood glucose levels and survival rate of Osphronemus gouramy seed in closed system transportation

The purpose of this research was to know the appropriation of extract from Datura metel L. seeds as anti-stress with ethanol solvent can give effect towards blood glucose levels and survival rate of Osphronemus Gouramy seed in closed system transportation, to know optimal doses of extract from Datura metel L. seeds as antistress with ethanol solvent can maintain normal blood glucose levels and yield the best survival rate of Osphronemus gouramy seed. This research was carried in September 2016 in Education Laboratory, Faculty of Fisheries and Marine Airlangga University Surabaya. This research used Completely Randomized Design with five treatments (negative control without extract, positive control with chloralhydrate, Datura metel L. seeds extract 0.5 g/L, 1,5 g/L dan 4.5 g/L) and four replications. Statistical analysis used the Analysis of Variant (ANOVA) to determine the effect of treatment and continued with Duncan's multiple range test. The results showed that the appropriation of extract from Datura metel L. seeds give effect towards blood glucose levels and survival rate on Osphronemus Gouramy seed. Doses 0.5 g/L was the optimal dose as antistress of Osphronemus Gouramy seed during closed system transportation.