Acid-Fast Staining Revisited, a Dated but Versatile Means of Diagnosis

Flora Ramona Sigit Prakoeswa, Bayu Bijaksana Rumondor, Cita Rosita Sigit Prakoeswa

Research output: Contribution to journalReview articlepeer-review


Acid-fast staining has been utilized in the field of medicine since the late 1800s. It was, for a while, considered the gold standard for diagnosing leprosy and tuberculosis. Principally it uses 1 primary staining agent, carbol fuchsin; and methylene blue as a counter stain. The emergence of molecular-based diagnostic tests makes some of us question the relevance of acid-fast staining in daily practice. This literature review aimed to revisit the acid-fast staining method, its uses, and the modification that occurred throughout the years.

Original languageEnglish
Article numbere187428582206081
JournalOpen Microbiology Journal
Issue number1
Publication statusPublished - 2022


  • Acid-fast staining
  • Bacteriological examination
  • Kinyoun
  • Staining protocol
  • Truant
  • Ziehl-Neelsen


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