TY - JOUR
T1 - Acid-Fast Staining Revisited, a Dated but Versatile Means of Diagnosis
AU - Prakoeswa, Flora Ramona Sigit
AU - Rumondor, Bayu Bijaksana
AU - Prakoeswa, Cita Rosita Sigit
N1 - Publisher Copyright:
© 2022 Prakoeswa et al.
PY - 2022
Y1 - 2022
N2 - Acid-fast staining has been utilized in the field of medicine since the late 1800s. It was, for a while, considered the gold standard for diagnosing leprosy and tuberculosis. Principally it uses 1 primary staining agent, carbol fuchsin; and methylene blue as a counter stain. The emergence of molecular-based diagnostic tests makes some of us question the relevance of acid-fast staining in daily practice. This literature review aimed to revisit the acid-fast staining method, its uses, and the modification that occurred throughout the years.
AB - Acid-fast staining has been utilized in the field of medicine since the late 1800s. It was, for a while, considered the gold standard for diagnosing leprosy and tuberculosis. Principally it uses 1 primary staining agent, carbol fuchsin; and methylene blue as a counter stain. The emergence of molecular-based diagnostic tests makes some of us question the relevance of acid-fast staining in daily practice. This literature review aimed to revisit the acid-fast staining method, its uses, and the modification that occurred throughout the years.
KW - Acid-fast staining
KW - Bacteriological examination
KW - Kinyoun
KW - Staining protocol
KW - Truant
KW - Ziehl-Neelsen
UR - http://www.scopus.com/inward/record.url?scp=85138607001&partnerID=8YFLogxK
U2 - 10.2174/18742858-v16-e2206081
DO - 10.2174/18742858-v16-e2206081
M3 - Review article
AN - SCOPUS:85138607001
SN - 1874-2858
VL - 16
JO - Open Microbiology Journal
JF - Open Microbiology Journal
IS - 1
M1 - e187428582206081
ER -