Tooth extraction can be debilitating due to prolonged inflammation delaying wound healing. Secretory leukocyte protease inhibitor (SLPI) is a non-glycosylated proteomic material that inhibits protease enzymes and has anti-inflammatory properties accelerating wound healing. Increased osteoclast activity is marked by increased osteopontin expression (OPN) during bone resorption. OPN is a phosphorylated glycoprotein secreted by several immune cells. This study aims to determine OPN's expression as an osteoclast activity's overview in wound healing acceleration, following SLPI administration. Thirty-six Rattus norvegicus incisors were extracted and randomly divided into 6 groups by treatment type and duration of observation. SLPI was administered in Group 1 and 2 (12 heads respectively) on the socket of rat's teeth, followed by tight stitches. Group 2 additionally received bone graft. Control group K (12 heads) exclusively received tooth socket stitching. Two observations, each on 6 rats, were performed at 7 and 14 days. Subsequently, biopsies were performed and immunohisto-chemically stained with osteopontin antibodies. One-way Anova and Kruskal-Wallis tests showed statistically significant differences (p<0.05) between the groups. Results suggest that SLPI administration decreases osteoclast activity in alveolar bone resorption by decreasing the number of active osteoclasts and increasing the number of inactive ones in a Rattus model. Osteopontin expression can be used in overview of osteoclast's activity.
|Journal||Journal of Physics: Conference Series|
|Publication status||Published - 7 Sept 2018|
|Event||2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018 - Depok, West Java, Indonesia|
Duration: 18 Jul 2018 → 18 Jul 2018