ACC1 and ACC2 Genes Expression Microalgae Skletonema costatum on Stress Aeration and fotoperiode in Simultans

Tutik Nurhidayati, Hery Purnobasuki, Wirdatul Muslihatin

Research output: Contribution to journalConference articlepeer-review

Abstract

Abiotic stresses such as photoperiod, aeration, CO2 concentration, pH, temperature, and salinity can affect and salinity the physiological processes of microalgae. One of the physiological processes that occur is a change in the lipid biosynthetic pathway towards the formation and accumulation of neutral lipids. Neutral lipid accumulation in the form of TAGs. TAGs as one of the defense mechanisms. The regulation of fatty acid biosynthesis is initiated by the ACC1 and ACC2 genes. This study aims to determine the expression of ACC1 and ACC2 genes under conditions of aeration stress and photoperiod simultaneously in microalgae Skeletonema costatum. The method used was the analysis of gene expression in the sample of S. costatum RNA extract using qRT-PCR. Based on the results of the study, it was found that the ACC1 gene was expressed and upregulated in the A2F2 treatment (24-hour aeration and 24:0 photoperiod) with a CT value of 36.85 while the ACC2 gene was expressed and upregulated in the A1F1 treatment (12-hour aeration and 12:12 photoperiod). with the CT value of 36,874.

Original languageEnglish
Article number012013
JournalIOP Conference Series: Earth and Environmental Science
Volume1097
Issue number1
DOIs
Publication statusPublished - 2022
Event1st Lekantara Annual Conference on Natural Science and Environment, LeNS 2021 - Malang, Indonesia
Duration: 20 Sep 202121 Sep 2021

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