TY - JOUR
T1 - A potential therapy of human umbilical cord mesenchymal stem cells for bone regeneration on osteoporotic mandibular bone
AU - Hendrijantini, Nike
AU - Kusumaningsih, Tuti
AU - Rostiny, Rostiny
AU - Mulawardhana, Pungky
AU - Danudiningrat, Coen Pramono
AU - Rantam, Fedik Abdul
N1 - Publisher Copyright:
© 2018 European Journal of Dentistry | Published by Wolters Kluwer - Medknow.
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Objective: The aim of this study is to prove that human umbilical cord mesenchymal stem cell (hUCMSC) therapy on mandibular osteoporotic model is able to increase transforming growth factor-beta-1 (TGF)-β1 expression, Runx2, and osteoblasts. Materials and Methods: This research is true experimental posttest control group design. Thirty female Wistar rats were divided into 6 groups randomly, which consisted of sham surgery for control (T1), ovariectomy as osteoporotic group (T2), osteoporotic group injected with gelatine for 4 weeks (T3), 8 weeks (T4) injected with hUCMSC-gelatine for 4 weeks (T5) and 8 weeks (T6). All mice were presented for immunohistochemistry examination for TGF-β1, Runx2, and histology for osteoblasts. Results: The lowest level of osteoblast was osteoporotic group injected with gelatine in 4 weeks compared to other groups. There were increases of TGF-β1, Runx2, and osteoblasts from osteoporotic group compared to osteoporotic post-hUCMSC-gelatine injection group. Conclusion: The hUCMSC has a high osteogenic effect and increases the osteoporotic mandibular bone regeneration on the animal model that is showed by the increase of the level of TGF-β1, Runx2, and osteoblasts.
AB - Objective: The aim of this study is to prove that human umbilical cord mesenchymal stem cell (hUCMSC) therapy on mandibular osteoporotic model is able to increase transforming growth factor-beta-1 (TGF)-β1 expression, Runx2, and osteoblasts. Materials and Methods: This research is true experimental posttest control group design. Thirty female Wistar rats were divided into 6 groups randomly, which consisted of sham surgery for control (T1), ovariectomy as osteoporotic group (T2), osteoporotic group injected with gelatine for 4 weeks (T3), 8 weeks (T4) injected with hUCMSC-gelatine for 4 weeks (T5) and 8 weeks (T6). All mice were presented for immunohistochemistry examination for TGF-β1, Runx2, and histology for osteoblasts. Results: The lowest level of osteoblast was osteoporotic group injected with gelatine in 4 weeks compared to other groups. There were increases of TGF-β1, Runx2, and osteoblasts from osteoporotic group compared to osteoporotic post-hUCMSC-gelatine injection group. Conclusion: The hUCMSC has a high osteogenic effect and increases the osteoporotic mandibular bone regeneration on the animal model that is showed by the increase of the level of TGF-β1, Runx2, and osteoblasts.
KW - Human umbilical cord mesenchymal stem cells
KW - mandibular
KW - osteoporosis
UR - http://www.scopus.com/inward/record.url?scp=85051250051&partnerID=8YFLogxK
U2 - 10.4103/ejd.ejd_342_17
DO - 10.4103/ejd.ejd_342_17
M3 - Article
AN - SCOPUS:85051250051
SN - 1305-7456
VL - 12
SP - 358
EP - 362
JO - European Journal of Dentistry
JF - European Journal of Dentistry
IS - 3
ER -