A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature

Abdul Wafi; Ganden Supriyanto; Tjitjik Srie Tjahjandarie

doi:10.22146/ijc.21174

A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature

Abdul Wafi, Ganden Supriyanto, Tjitjik Srie Tjahjandarie

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO₂, bismuth nitrate pentahydrate (Bi(NO₃)₃.5H₂O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 μg/mL and 8-9 min respectively.

Original language	English
Pages (from-to)	32-35
Number of pages	4
Journal	Indonesian Journal of Chemistry
Volume	16
Issue number	1
DOIs	https://doi.org/10.22146/ijc.21174
Publication status	Published - 2016

Keywords

Azo dye
Chloramphenicol
Diazotization reaction
Spectrophotometry

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title = "A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature",

abstract = "An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO2, bismuth nitrate pentahydrate (Bi(NO3)3.5H2O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 μg/mL and 8-9 min respectively.",

keywords = "Azo dye, Chloramphenicol, Diazotization reaction, Spectrophotometry",

author = "Abdul Wafi and Ganden Supriyanto and Tjahjandarie, {Tjitjik Srie}",

year = "2016",

doi = "10.22146/ijc.21174",

language = "English",

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T1 - A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature

AU - Wafi, Abdul

AU - Supriyanto, Ganden

AU - Tjahjandarie, Tjitjik Srie

PY - 2016

Y1 - 2016

N2 - An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO2, bismuth nitrate pentahydrate (Bi(NO3)3.5H2O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 μg/mL and 8-9 min respectively.

AB - An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO2, bismuth nitrate pentahydrate (Bi(NO3)3.5H2O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 μg/mL and 8-9 min respectively.

KW - Azo dye

KW - Chloramphenicol

KW - Diazotization reaction

KW - Spectrophotometry

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U2 - 10.22146/ijc.21174

DO - 10.22146/ijc.21174

M3 - Article

AN - SCOPUS:84962356010

SN - 1411-9420

VL - 16

SP - 32

EP - 35

JO - Indonesian Journal of Chemistry

JF - Indonesian Journal of Chemistry

IS - 1

ER -

A novel spectrophotometric method for determination of chloramphenicol based on diazotization reaction at room temperature

Abstract

Keywords

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