A novel method of sampling gingival crevicular fluid from a mouse model of periodontitis

Shinji Matsuda, Alexandru Movila, Maiko Suzuki, Mikihito Kajiya, Wichaya Wisitrasameewong, Rayyan Kayal, Josefine Hirshfeld, Ayman Al-dharrab, Irma J. Savitri, Abdulghani Mira, Hidemi Kurihara, Martin A. Taubman, Toshihisa Kawai

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


Using a mouse model of silk ligature-induced periodontal disease (PD), we report a novel method of sampling mouse gingival crevicular fluid (GCF) to evaluate the time-dependent secretion patterns of bone resorption-related cytokines. GCF is a serum transudate containing host-derived biomarkers which can represent cellular response in the periodontium. As such, human clinical evaluations of PD status rely on sampling this critical secretion. At the same time, a method of sampling GCF from mice is absent, hindering the translational value of mouse models of PD. Therefore, we herein report a novel method of sampling GCF from a mouse model of periodontitis, involving a series of easy steps. First, the original ligature used for induction of PD was removed, and a fresh ligature for sampling GCF was placed in the gingival crevice for 10 min. Immediately afterwards, the volume of GCF collected in the sampling ligature was measured using a high precision weighing balance. The sampling ligature containing GCF was then immersed in a solution of PBS-Tween 20 and subjected to ELISA. This enabled us to monitor the volume of GCF and detect time-dependent changes in the expression of such cytokines as IL-1b, TNF-α, IL-6, RANKL, and OPG associated with the levels of alveolar bone loss, as reflected in GCF collected from a mouse model of PD. Therefore, this novel GCF sampling method can be used to measure various cytokines in GCF relative to the dynamic changes in periodontal bone loss induced in a mouse model of PD.

Original languageEnglish
Pages (from-to)21-25
Number of pages5
JournalJournal of Immunological Methods
Publication statusPublished - 1 Nov 2016


  • Gingival crevicular fluid
  • Ligature-induced periodontal disease
  • Mice
  • Periodontal disease


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