A novel immunochromatographic system for easy-to-use detection of group 1 avian influenza viruses with acquired human-type receptor binding specificity

Yohei Watanabe, Tetsuo Ito, Madiha S. Ibrahim, Yasuha Arai, Kozue Hotta, Hoang Vu Mai Phuong, Nguyen Le Khanh Hang, Le Quynh Mai, Kosuke Soda, Masaoki Yamaoka, Emmanuel Djoko Poetranto, Laksmi Wulandari, Hiroaki Hiramatsu, Tomo Daidoji, Ritsuko Kubota-Koketsu, Nongluk Sriwilaijaroen, Takaaki Nakaya, Yoshinobu Okuno, Tadanobu Takahashi, Takashi SuzukiToshihiro Ito, Hak Hotta, Tetsu Yamashiro, Tsukasa Hayashi, Kouichi Morita, Kazuyoshi Ikuta, Yasuo Suzuki

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

A switch of viral hemagglutinin receptor binding specificity from bird-type α2,3- to human-type α2,6-linked sialic acid is necessary for an avian influenza virus to become a pandemic virus. In this study, an easy-to-use strip test to detect receptor binding specificity of influenza virus was developed. A biotinylated anti-hemagglutinin antibody that bound a broad range of group 1 influenza A viruses and latex-conjugated α2,3 (blue) and α2,6 (red) sialylglycopolymers were used in an immunochromatographic strip test, with avidin and lectin immobilized on a nitrocellulose membrane at test and control lines, respectively. Accumulation of a sialylglycopolymer-virus-antibody complex at the test line was visualized by eye. The strip test could be completed in 30. min and did not require special equipment or skills, thereby avoiding some disadvantages of current methods for analyzing receptor binding specificity of influenza virus. The strip test could detect the receptor binding specificity of a wide range of influenza viruses, as well as small increases in the binding affinity of variant H5N1 viruses to α2,6 sialylglycans at viral titers >128 hemagglutination units. The strip test results were in agreement with those of ELISA virus binding assays, with correlations >0.95. In conclusion, the immunochromatographic strip test developed in this study should be useful for monitoring potential changes in the receptor binding specificity of group 1 influenza A viruses in the field.

Original languageEnglish
Pages (from-to)211-219
Number of pages9
JournalBiosensors and Bioelectronics
Volume65
DOIs
Publication statusPublished - 5 Mar 2015

Keywords

  • Detection of receptor binding specificity
  • Group 1 influenza A virus
  • H5N1 avian influenza virus
  • Immunochromatographic strip test
  • Pandemic potential
  • Sialylglycopolymer

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