A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical

Tin Myo Thant; Nanik Siti Aminah; Alfinda Novi Kristanti; Rico Ramadhan; Preecha Phuwapraisirisan; Yoshiaki Takaya

doi:10.1080/14786419.2019.1586696

A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical

Tin Myo Thant, Nanik Siti Aminah, Alfinda Novi Kristanti, Rico Ramadhan, Preecha Phuwapraisirisan, Yoshiaki Takaya

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC₅₀ values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC₅₀ 5.45 µM) and sucrase (IC₅₀ 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC₅₀ values 1.92 and 5.58 mM.

Original language	English
Pages (from-to)	556-561
Number of pages	6
Journal	Natural Product Research
Volume	35
Issue number	4
DOIs	https://doi.org/10.1080/14786419.2019.1586696
Publication status	Published - 2021

Keywords

Clausena excavata
IC50
antioxidant
inhibition
pyrano coumarin
α-glycosidase

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10.1080/14786419.2019.1586696

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title = "A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical",

abstract = "A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC50 values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC50 5.45 µM) and sucrase (IC50 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC50 values 1.92 and 5.58 mM.",

keywords = "Clausena excavata, IC50, antioxidant, inhibition, pyrano coumarin, α-glycosidase",

author = "Thant, {Tin Myo} and Aminah, {Nanik Siti} and Kristanti, {Alfinda Novi} and Rico Ramadhan and Preecha Phuwapraisirisan and Yoshiaki Takaya",

note = "Funding Information: T.M.T. would like to thank Universitas Airlangga, Directorate General of Higher Education (DIKTI), Ministry of Education and Culture of Indonesia that has funded this research. Publisher Copyright: {\textcopyright} 2019 Informa UK Limited, trading as Taylor & Francis Group.",

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doi = "10.1080/14786419.2019.1586696",

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AU - Thant, Tin Myo

AU - Aminah, Nanik Siti

AU - Kristanti, Alfinda Novi

AU - Ramadhan, Rico

AU - Phuwapraisirisan, Preecha

AU - Takaya, Yoshiaki

N1 - Funding Information: T.M.T. would like to thank Universitas Airlangga, Directorate General of Higher Education (DIKTI), Ministry of Education and Culture of Indonesia that has funded this research. Publisher Copyright: © 2019 Informa UK Limited, trading as Taylor & Francis Group.

PY - 2021

Y1 - 2021

N2 - A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC50 values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC50 5.45 µM) and sucrase (IC50 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC50 values 1.92 and 5.58 mM.

AB - A new pyrano coumarin, identified as excavatin A (1) together with two known compounds nordentatin (2) and binorpocitrin (3) was isolated from the 95% EtOH extract of Clausena excavata. All structures were elucidated by using spectroscopy methods such as extensive NMR and HR-FAB-MS spectrometry. All the isolated compounds were tested on antidiabetes activity by using α-glucosidase inhibition assay and the antioxidant activity by DPPH assay. Compounds 1-3 showed antioxidant activity with IC50 values 0.286, 0.02, 0.278 mM. Among them, 2 exhibited inhibition activity against maltase (IC50 5.45 µM) and sucrase (IC50 43.57 µM). However, compounds (1) and (3) displayed inhibition on yeast α-glucosidase with IC50 values 1.92 and 5.58 mM.

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A new pyrano coumarin from Clausena excavata roots displaying dual inhibition against α-glucosidase and free radical

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Keywords

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